| Publication Type |
Journal Article |
| Title |
Novel biosensing device for point-of-care applications with plastic antibodies grown on Au-screen printed electrodes |
| Authors |
Felismina T. C. Moreira Rosa A. F. Dutra Joao Noronha JCS Fernandes M. Goreti F. Sales |
| Groups |
|
| Journal |
SENSORS AND ACTUATORS B-CHEMICAL |
| Year |
2013 |
| Month |
June |
| Volume |
182 |
| Number |
|
| Pages |
733-740 |
| Abstract |
A gold screen printed electrode (Au-SPE) was modified by merging Molecular Imprinting and Self-Assembly Monolayer techniques for fast screening cardiac biomarkers in point-of-care (POC). For this purpose, Myoglobin (Myo) was selected as target analyte and its plastic antibody imprinted over a glutaraldehyde (Glu)/cysteamine (Cys) layer on the gold-surface. The imprinting effect was produced by growing a reticulated polymer of acrylamide (AAM) and N,N -methylenebisacrylamide (NNMBA) around the Myo template, covalently attached to the biosensing surface. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) studies were carried out in all chemical modification steps to confirm the surface changes in the Au-SPE. The analytical features of the resulting biosensor were studied by different electrochemical techniques, including EIS, square wave voltammetry (SWV) and potentiometry. The limits of detection ranged from 0.13 to 8 mu g/mL. Only potentiometry assays showed limits of detection including the cut-off Myo levels. Quantitative information was also produced for Myo concentrations >= 0.2 mu g/mL. The linear response of the biosensing device showed an anionic slope of similar to 70 mVper decade molar concentration up to 0.3 mu g/mL. The interference of coexisting species was tested and good selectivity was observed. The biosensor was successfully applied to biological fluids. (C) 2013 Elsevier B.V. All rights reserved. |
| DOI |
http://dx.doi.org/10.1016/j.snb.2013.03.099 |
| ISBN |
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| Publisher |
|
| Book Title |
|
| ISSN |
0925-4005 |
| EISSN |
|
| Conference Name |
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| Bibtex ID |
ISI:000319488800099 |
| Observations |
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