| Publication Type |
Journal Article |
| Title |
Glutathione metabolism in hepatomous liver of rats treated with diethylnitrosamine |
| Authors |
H. Susana Marinho M Baptista RE Pinto |
| Groups |
|
| Journal |
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE |
| Year |
1997 |
| Month |
April |
| Volume |
1360 |
| Number |
2 |
| Pages |
157-168 |
| Abstract |
Glutathione metabolism was studied in rat liver during diethylnitrosamine (DEN) carcinogenesis. Some studies were also made in foetal rat liver. Endogenous GSH and non-protein thiols concentrations are increased in DEN-treated rats when compared to non-treated rats but no differences were found in cysteine, total thiols and protein thiols concentration. In foetal liver GSH concentration is only 35\% of that in DEN-treated rat liver. The activities of several enzymes involved in glutathione metabolism are changed in DEN-treated rats. gamma-Glutamyl transferase activity and cysteine formation from GSH by liver homogenates is increased sevenfold. gamma-Glutamylcysteine synthetase activity, initial rate of [S-35]cysteine incorporation in gamma-glutamylcysteine and initial rate of GSH formation from [S-35]cysteine are increased two-fold. Cytosolic GSH S-transferase activity is increased twofold in DEN-treated rats and so GSH S-conjugates concentration is probably also increased. In foetal rat liver gamma-glutamyl transferase activity is about the same but gamma-glutamylcysteine synthetase activity is only 10\% of that in DEN-treated rat liver. The increased GSH concentration in DEN-treated rat liver is probably due to the simultaneous increase in the activities of gamma-glutamyl transferase and gamma-glutamylcysteine synthetase. Blood plasma total glutathione is increased 1.4 times in DEN-treated rats, but no differences are found in GSH hepatic arteriovenous gradient. This associated with the increased gamma-glutamyl transferase activity suggests that sinusoidal GSH efflux is increased in DEN-treated rats. |
| DOI |
http://dx.doi.org/10.1016/S0925-4439(96)00075-0 |
| ISBN |
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| Publisher |
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| Book Title |
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| ISSN |
0925-4439 |
| EISSN |
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| Conference Name |
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| Bibtex ID |
ISI:A1997WV13900007 |
| Observations |
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