Publication Type Journal Article
Title Enhanced antibacterial activity of Rosehip extract-functionalized Mg(OH)(2) nanoparticles: An in vitro and in vivo study
Authors Marta M. Alves Catarina Batista Dalila Mil-Homens Liliana Grenho Maria H. Fernandes C. Santos
Groups CSSE
Journal COLLOIDS AND SURFACES B-BIOINTERFACES
Year 2022
Month
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Volume 217
Number
Pages
Abstract The development of nanoparticles as antimicrobial agents against pathogenic bacteria has emerged as one of the leading global healthcare challenges. In this study, Mg(OH)2 NPs with controlled morphology and nanometric size, using two distinct counterions, chloride or nitrate, have been synthesized using Rosehip (RH) extract that has privileges beyond conventional chemical and physical methods. Various physicochemical techniques were used to characterize the RH-functionalized Mg-based NPs. They exhibited a spherical shape with a diameter of \~10 nm, low crystallinity compared to non-functionalized NPs, high polyphenol content, and negative zeta potential in three different media (H2O, TSB, and cell medium). The resulting RH-functionalized Mg-based NPs also exhibited an increased antibacterial activity against Gram-positive (S. Epidermis and S. aureus) and Gramnegative (E. Coli) bacteria compared to those prepared in pure water (0 \% RH), an effect that was well evident with low NPs contents (250 mu g/mL). A preliminary attempt to elucidate their mechanism of action revealed that RH-functionalized Mg-based NPs could disrupt cellular structures (bacterial cell wall and cytoplasmic membrane) and damage the bacterial cell, as confirmed by TEM imaging. Noteworthy is that Mg-based NPs exhibited higher toxicity to bacteria than to eukaryotic cells. More significantly, was their enhanced in vivo efficacy in a Galleria mellonella invertebrate animal model, when infected with S. aureus bacteria. Overall, our findings indicate that well-engineered Rosehip magnesium-based nanoparticles can be used as a green noncytotoxic polyphenolic source in different antibacterial applications for the biomedical industry.
DOI http://dx.doi.org/10.1016/j.colsurfb.2022.112643
ISBN
Publisher ELSEVIER
Book Title
ISSN 0927-7765
EISSN 1873-4367
Conference Name
Bibtex ID WOS:000827610900002
Observations
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