| Publication Type |
Journal Article |
| Title |
Development of a Topical Insulin Polymeric Nanoformulation for Skin Burn Regeneration: An Experimental Approach |
| Authors |
Maria Quiterio Sandra Simoes Andreia Ascenso Manuela C. Carvalheiro Ana Paula Leandro Isabel Correia A. Viana Pedro Faisca Lia Ascensao Jesus Molpeceres M. Manuela Gaspar Catarina Pinto Reis |
| Groups |
BIOIN Chem4Env |
| Journal |
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
| Year |
2021 |
| Month |
April |
| Volume |
22 |
| Number |
8 |
| Pages |
|
| Abstract |
Insulin is a peptide hormone with many physiological functions, besides its use in diabetes treatment. An important role of insulin is related to the wound healing process-however, insulin itself is too sensitive to the external environment requiring the protective of a nanocarrier. Polymer-based nanoparticles can protect, deliver, and retain the protein in the target area. This study aims to produce and characterize a topical treatment for wound healing consisting of insulin-loaded poly-DL-lactide/glycolide (PLGA) nanoparticles. Insulin-loaded nanoparticles present a mean size of approximately 500 nm and neutral surface charge. Spherical shaped nanoparticles are observed by scanning electron microscopy and confirmed by atomic force microscopy. SDS-PAGE and circular dichroism analysis demonstrated that insulin preserved its integrity and secondary structure after the encapsulation process. In vitro release studies suggested a controlled release profile. Safety of the formulation was confirmed using cell lines, and cell viability was concentration and time-dependent. Preliminary safety in vivo assays also revealed promising results. |
| DOI |
http://dx.doi.org/10.3390/ijms22084087 |
| ISBN |
|
| Publisher |
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| Book Title |
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| ISSN |
|
| EISSN |
1422-0067 |
| Conference Name |
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| Bibtex ID |
ISI:000644347900001 |
| Observations |
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