Publication Type Journal Article
Title Copper Complexes with 1,10-Phenanthroline Derivatives: Underlying Factors Affecting Their Cytotoxicity
Authors Patrique Nunes Isabel Correia Fernanda M. Marques Antonio Pedro Matos Margarida Correia dos Santos Cristina G. Azevedo Jose-Luis Capelo Hugo M. Santos Sofia Gama Teresa Pinheiro Isabel Cavaco J.C. Pessoa
Groups BIOIN Chem4Env
Journal INORGANIC CHEMISTRY
Year 2020
Month July
Volume 59
Number 13
Pages 9116-9134
Abstract The interpretation of in vitro cytotoxicity data of Cu(II)-1,10-phenanthroline (phen) complexes normally does not take into account the speciation that complexes undergo in cell incubation media and its implications in cellular uptake and mechanisms of action. We synthesize and test the activity of several distinct Cu(II)-phen compounds; up to 24 h of incubation, the cytotoxic activity differs for the Cu complexes and the corresponding free ligands, but for longer incubation times (e.g., 72 h), all compounds display similar activity. Combining the use of several spectroscopic, spectrometric, and electrochemical techniques, the speciation of Cu-phen compounds in cell incubation media is evaluated, indicating that the originally added complex almost totally decomposed and that Cu(II) and phen are mainly bound to bovine serum albumin. Several methods are used to disclose relationships between structure, activity, speciation in incubation media, cellular uptake, distribution of Cu in cells, and cytotoxicity. Contrary to what is reported in most studies, we conclude that interaction with cell components and cell death involves the separate action of Cu ions and phen molecules, not [Cu(phen)(n)] species. This conclusion should similarly apply to many other Cu-ligand systems reported to date.
DOI http://dx.doi.org/10.1021/acs.inorgchem.0c00925
ISBN
Publisher AMER CHEMICAL SOC
Book Title
ISSN 0020-1669
EISSN 1520-510X
Conference Name
Bibtex ID ISI:000548456300051
Observations
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