Publication Type Journal Article
Title Standard addition method with cumulative spikes: uric acid determination in human serum by voltammetry with optimized uncertainty
Authors Tony R. L. Dadamos Airton J. Damaceno Fernando L. Fertonani Ricardo Bettencourt da Silva
Groups Chem4Env
Journal ACCREDITATION AND QUALITY ASSURANCE
Year 2018
Month December
Volume 23
Number 6
Pages 337-348
Abstract This work presents a low-cost, simple and adequately reliable voltammetric alternative to the determination of uric acid (UA) in human serum by the enzymatic colorimetric standard method. UA is an organic molecule of great biomedical interest since its level is used in the diagnosis of several diseases. The quality of the electrochemical measurements was evaluated by comparing their expandeduncertainty with a target (i.e. maximum admissible) value of 0.56mgdL(-1) (one eighth of UA ranges of healthy individuals) and by analysing spiked serum and human serum samples measured by the standard method. The modified working electrode has 25\% lignin, 60\% nanocarbon, 15\% mineral oil and a copper electrodeposit. Due to the vulnerability of voltammetric techniques to matrix effects and to the small amount of serum samples, it is used the standard addition method with cumulative analyte additions for electrode calibration. The measurement uncertainty was estimated by the bottom-up approach where uncertainty components were combined using either the uncertainty propagation law or the numerical Kragten method. The analytical procedure was successfully applied to the analysis of physiological serums spiked at 1.0mgdL(-1), 3.0mgdL(-1), 5.0mgdL(-1), 7.0mgdL(-1) or 9.0mgdL(-1) of UA and two human serum samples. The results of analysed serums estimated by the voltammetric procedure have adequately low uncertainty and are metrologically equivalent (i.e. compatible) to the reference values estimated by the spiking process or by the standard method. Therefore, the developed voltammetric measurement procedure is a promising alternative to the standard procedure.
DOI http://dx.doi.org/10.1007/s00769-018-1350-8
ISBN
Publisher
Book Title
ISSN 0949-1775
EISSN 1432-0517
Conference Name
Bibtex ID ISI:000450983100003
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