Publication Type Journal Article
Title High throughput bar adsorptive microextraction: A novel cost-effective tool for monitoring benzodiazepines in large number of biological samples
Authors S. M. E. Ahmad J. M. F. Nogueira
Groups Chem4Env
Journal TALANTA
Year 2019
Month July
Volume 199
Number
Pages 195-202
Abstract In this work, we propose an innovative high throughput (HT) apparatus using the bar adsorptive microextraction (BA mu E) technique, which enables the simultaneous enrichment of up to 100 samples. This novel configuration was combined with microliquid desorption and high-performance liquid chromatography-diode array detection to monitor trace levels of eight benzodiazepines (diazepam, prazepam, bromazepam, oxazepam, lorazepam, alprazolam, temazepam and loflazepate) in biological samples. The proposed methodology was fully developed, optimized and validated, resulting in suitable intraday and interday precision (RSD <= 15\%), with recovery yields ranging from 33.0\% to 104.5\%. The lower limits of quantification were between 20.0 and 100.0 mu g L-1, using 1.0 mL of urine and 0.5 mL of plasma or serum samples. The application of the proposed methodology to real matrices resulted in average sample preparation time of around 2 min per sample, demonstrating that it is user-friendly, cost-effective and a rapid decision-making tool, whenever large number of samples are involved.
DOI http://dx.doi.org/10.1016/j.talanta.2019.02.004
ISBN
Publisher
Book Title
ISSN 0039-9140
EISSN 1873-3573
Conference Name
Bibtex ID ISI:000465050000027
Observations
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