Publication Type Journal Article
Title Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles
Authors A.P.C. Ribeiro S. Anbu E. C. B. A. Alegria A. R. Fernandes P. V. Baptista R. Mendes A. S. Matias M. Mendes M. F. C. Guedes da Silva A. J. L. Pombeiro
Groups CCC
Journal BIOMEDICINE \& PHARMACOTHERAPY
Year 2018
Month May
Volume 101
Number
Pages 137-144
Abstract Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 +/- 0.1 mu M) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 +/- 0.1 mu M). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.
DOI http://dx.doi.org/10.1016/j.biopha.2018.02.069
ISBN
Publisher
Book Title
ISSN 0753-3322
EISSN 1950-6007
Conference Name
Bibtex ID ISI:000429586400017
Observations
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