Publication Type Journal Article
Title M13 bacteriophage purification using poly(ionic liquids) as alternative separation matrices
Authors Maria Joao Jacinto David J. S. Patinha Isabel Marrucho Joao Goncalves Richard C. Willson Ana M. Azevedo M. Raquel Aires-Barros
Groups MET
Journal JOURNAL OF CHROMATOGRAPHY A
Year 2018
Month January
Volume 1532
Number
Pages 246-250
Abstract M13 is a filamentous, non-lytic bacteriophage that infects Escherichia coli via the F pilus. Currently, phage M13 is widely used in phage display technology and bio-nanotechnology, and is considered a possible antibacterial therapeutic agent, among other applications. Conventional phage purification involves 5-7 operational steps, with high operational costs and significant product loss (approximately 60\%). In this work, we propose a scalable purification process for M13 bacteriophage using a novel stationary phase based on a polymeric ionic liquid (PIL) with a positively charged backbone structure. Poly (1-vinyl-3-ethyl imidazolium bis(trifluoromethylsulfonyl) imide) - poly(VEIM-TFSI) predominantly acted as an anion exchanger under binding-elution mode. This revealed to be a rapid and simple method for the recovery of phage M13 with an overall separation yield of over 70\% after a single downstream step. To the best of our knowledge, PILs have never been used as separation matrices for biological products and the results obtained, together with the large number of cations and anions available to prepare PILs, illustrate well the large potential of the proposed methodology. (C) 2017 Elsevier B.V. All rights reserved.
DOI http://dx.doi.org/10.1016/j.chroma.2017.12.005
ISBN
Publisher
Book Title
ISSN 0021-9673
EISSN 1873-3778
Conference Name
Bibtex ID ISI:000423894500028
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