Abstract |
Homogeneous, glossy and transparent nanocomposite films composed of pullulan (PL) and lysozyme nanofibers (LNFs) were developed by simple solvent casting from aqueous suspensions. The incorporation of LNFs into the PL matrix maintained the filmogenic ability of PL and endowed the nanocomposite films with good mechanical properties (Young s modulus = 1.91-2.50 GPa) and several functionalities, confirming the potential of LNFs as bioactive reinforcing elements for nanocomposites. The set of pliable films exhibits thermal stability up to 225 degrees C and maximum antioxidant activity around 77\% (DPPH scavenging activity) for the film with the highest LNFs content (15.0 wt\%). The antibacterial activity of the ensuing nanocomposite films towards Staphylococcus aureus, viz. lysozyme-resistant bacteria, was assessed, and the data show augmented antibacterial effectiveness with increasing content of nanofibers. These promising results show the potential of PL/LNFs nanocomposites as eco-friendly edible films for active packaging. (C) 2017 Elsevier Ltd. All rights reserved. |