Publication Type Journal Article
Title Nanoparticulate vaccine inhibits tumor growth via improved T cell recruitment into melanoma and huHER2 breast cancer
Authors Eva Zupancic Caterina Curato Jung-Seok Kim Eilam Yeini Ziv Porat A. Viana Anat Globerson-Levin Tova Waks Zelig Eshhar Joao N. Moreira Ronit Satchi-Fainaro Lea Eisenbach Steffen Jung Helena F. Florindo
Groups
Journal NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Year 2018
Month April
Volume 14
Number 3
Pages 835-847
Abstract Nanoparticulate vaccines are promising tools to overcome cancer immune evasion. However, a deeper understanding on nanoparticle-immune cell interactions and treatments regime is required for optimal efficacy. We provide a comprehensive study of treatment schedules and mode of antigen-association to nanovaccines on the modulation of T cell immunity in vivo, under steady-state and tumor-bearing mice. The coordinated delivery of antigen and two adjuvants (Monophosphoryl lipid A, oligodeoxynucleotide cytosine-phosphate-guanine motifs (CpG)) by nanoparticles was crucial for dendritic cell activation. A single vaccination dictated a 3-fold increase on cytotoxic memory-T cells and raised antigen-specific immune responses against B16.M05 melanoma. It generated at least a 5-fold increase on IFN-gamma cytokine production, and presented over 50\% higher lymphocyte count in the tumor microenvironment, compared to the control. The number of lymphocytes at the tumor site doubled with triple immunization. This lymphocyte infiltration pattern was confirmed in mammary huHER2 carcinoma, with significant tumor reduction. (c) 2018 Elsevier Inc. All rights reserved.
DOI http://dx.doi.org/10.1016/j.nano.2017.12.011
ISBN
Publisher
Book Title
ISSN 1549-9634
EISSN 1549-9642
Conference Name
Bibtex ID ISI:000429528900019
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