Publication Type Journal Article
Title Therapeutic activity of superoxide dismutase-containing enzymosomes on rat liver ischaemia-reperfusion injury followed by magnetic resonance microscopy
Authors Paulo Marcelino H. Susana Marinho Maria Celeste Campos Ana Rita Neves Carla Real Filipa S. Fontes Alexandra Carvalho Gabriel Feio M. Barbara F. Martins M. L. Corvo
Groups
Journal EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
Year 2017
Month November
Volume 109
Number
Pages 464-471
Abstract Liver ischaemia-reperfusion injury (IRI) may occur during hepatic surgery and is unavoidable in liver transplantation. Superoxide dismutase enzymosomes (SOD-enzymosomes), liposomes where SOD is at the liposomal surface expressing enzymatic activity in intact form without the need of liposomal disruption, were developed with the aim of having a better insight into its antioxidant therapeutic outcome in IRI. We also aimed at validating magnetic resonance microscopy (MRM) at 7 T as a tool to follow IRI. SOD-enzymosomes were characterized and tested in a rat ischaemia-reperfusion model and the therapeutic outcome was compared with conventional long circulating SOD liposomes and free SOD using biochemical liver injury biomarkers, histology and MRM. MRM results correlated with those obtained using classical biochemical biomarkers of liver injury and liver histology. Moreover, MRM images suggested that the therapeutic efficacy of both SOD liposomal formulations used was related to prevention of peripheral biliary ductular damage and disrupted vascular architecture. Therefore, MRM at 7 T is a useful technique to follow IRI. SOD-enzymosomes were more effective than conventional liposomes in reducing liver ischaemia-reperfusion injury and this may be due to a short therapeutic window.
DOI http://dx.doi.org/10.1016/j.ejps.2017.09.008
ISBN
Publisher
Book Title
ISSN 0928-0987
EISSN 1879-0720
Conference Name
Bibtex ID ISI:000413325000048
Observations
Back to Publications List