Publication Type Journal Article
Title Encapsulation of photoactive porphyrinoids in polyelectrolyte hollow microcapsules viewed by fluorescence lifetime imaging microscopy (FLIM)
Authors Raquel Teixeira Vanda Vaz Serra Pedro M. R. Paulo Suzana M. Andrade Sílvia M. B. Costa
Groups MPPM
Journal RSC ADVANCES
Year 2015
Month
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Volume 5
Number 96
Pages 79050-79060
Abstract Fluorescence Lifetime Imaging Microscopy (FLIM) was used to investigate the encapsulation of porphyrinoids in multilayer hollow microcapsules assembled layer by layer with poly(styrene sulfonate) (PSS) and poly(allylamine hydrochloride) (PAH). The lifetime colour contrast enables the discrimination of fluorophore interactions at the microcapsule interface or deeper localized in the microcapsule shell. The coupling of functionalized porphyrin derivatives with oppositely charged polyelectrolyte made the microcapsule pH responsive. FLIM images were also obtained from aluminium monosulfonate phthalocyanine (AlPcS1) included in a lipid vesicle deposited on the surface of a modified microcapsule with dispersed gold nanoparticles (AuNP). In this case, a heterogeneous distribution of both quenched and enhanced fluorescence intensities was mapped from various fluorescence spots. These effects, undetected in the absence of AuNP, were accompanied by a decrease of lifetimes attributed to the contribution of plasmonic effects induced by AuNP. Fluorescence lifetime contrast-based imaging provides new insights in the field of polyelectrolyte microcapsules.
DOI http://dx.doi.org/10.1039/c5ra15504e
ISBN
Publisher ROYAL SOC CHEMISTRY
Book Title
ISSN 2046-2069
EISSN
Conference Name
Bibtex ID ISI:000361675800083
Observations
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